Handling and grading operations on trout farms cause acute physiological stress that can compromise welfare and production outcomes.
Repeated handling causes cumulative physiological stress in rainbow trout. Crowding for netting triggers a full stress response, elevating cortisol, suppressing immunity, and impairing gill function. Fish that are handled when already physiologically compromised (post-vaccination, during temperature extremes) are at higher welfare risk. Minimising handling frequency, using anaesthetic during necessary procedures, and investing in automated systems are evidence-based welfare improvements.