Octopuses, cuttlefish, and squid are increasingly used in research, raising welfare questions that new regulations are beginning to address.
The inclusion of cephalopods in EU research animal regulations was a recognition that their cognitive complexity warrants welfare consideration. However, welfare standards developed for vertebrates do not translate directly to cephalopods whose needs and stress responses differ fundamentally. Developing cephalopod-specific welfare guidance, including appropriate housing conditions, enrichment requirements, and humane endpoints, is a priority as their research use grows.