Rainbow trout are one of the most commonly farmed freshwater fish, with crowding stress being the primary welfare challenge in commercial production systems.
Crowding stress in rainbow trout creates a cascade of welfare problems: social competition for space, reduced ability to avoid aversive stimuli, compromised immune function, and chronic physiological stress. Fish at high densities spend more time in oxygen-depleted boundary layers near crowded conspecifics. Regulatory density limits based on welfare evidence rather than purely on mortality thresholds would better protect trout welfare.